Service Description
To date, most proteomics studies have been based on proteins obtained from the homogenization of large volumes of cells or tissues. However, with the advancement of medical research, there is a growing need for the analysis of highly specialized specimens within specific applications and research areas such as trace specimens identified by forensic experts, or intra-tumor heterogeneity research.
Nanoproteomics refers to quantitative proteomics analysis of small cell populations (typically < 5,000 cells) by the combination of in-situ cleavage and label free technology, and offers possibilities for analysis unavailable with conventional protein extraction and mass spectrometry.
BGI Genomics has extensive experience in the field of nanoproteomics spanning cellular heterogeneity research across rare cell populations, hard-to-obtain clinical specimens, and pathological tissues.
Project Workflow
References
[1] L. Yi, et al., Advances in microscale separations towards nanoproteomics applications, 2017.1523: 40–48.
How to order
Mass Spectrometry Service Specification
Sample Preparation and Services
- The QC of enzymatic hydrolysis is strictly controlled by LC-MS
- Different mass spectrometry, like Orbitrap Astral, timsTOF Pro, Orbitrap Fusion Lumos, et al, can be used
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
- Typical 5-6 weeks from sample QC acceptance to data report delivery
Sample Requirements
- Protein identification and quantification list
- Cluster analysis of expression patterns across multiple samples
- Protein GO/COG/KOG/Pathway annotation
- GO/Pathway enrichment analysis of differential proteins
- Protein-protein interaction analysis
- Protein subcellular localization analysis
- Repeatability analysis
- Quantitative proteomics and phosphoproteomics correlation analysis
Resources
