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    Label-free DDA Proteomics

    DESCRIPTION
    SPECIFICATION
    RESOURCES

    Project Workflow

    • PROTEIN EXTRACTION
    • PROTEIN DIGESTION
    • HPLC FRACTIONATION
    • LC-MS/MS (DDA)
    • DATA ANALYSIS

    Mass Spectrometry Service Specification

    Label-free DDA Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Triple TOF 5600).
    • Sample Preparation and Services

      • The QC of enzymatic hydrolysis is strictly controlled by LC-MS
      • 2h for DDA analysis
    • Quality Standard

      • Summary includes all methods and data analysis
      • Reports provided in Excel or PDF format, RAW files available upon request
    • Turn Around Time

      • Typical 3-4 weeks from sample QC acceptance to data report delivery

    Sample Requirements

    Data Analysis

    DATA ANALYSIS
    • Protein identification and quantification list
    • Differential proteins data statistics and volcano plot
    • Principal component analysis (PCA) and cluster analysis
    • Protein GO/COG/KOG/Pathway annotation
    • GO/COG/KOG enrichment analysis of differential proteins
    • Protein-protein interaction analysis
    • Protein subcellular localization analysis
    • Time series analysis
    • Repeatability analysis

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